Description
This sampler kit includes three imaging probes with different spectroscopic properties and high, medium and low bone affinities (in the order of 5(6)-ROX-RIS > 5(6)-FAM-dRIS > AF647-RISPC). All three probes include bisphosphonate or phosphonocarboxylate molecular skeleton, thus have higher affinity than calcein (the fluorescent molecule with the least bone affinity). The kit can be used for simultaneous detection with co-dosing and studies on effects of bone affinity.
Spectroscopic properties of 5(6)-ROX-RIS:
Maximum absorption wavelength (λmax (abs)): 580 nm
Maximum emission wavelength (λmax (em)): 605 nm
Extinction coefficient (M-1cm-1): 73,000 (0.1 M phosphate buffer, pH 8.0)
Spectroscopic properties of 5(6)-FAM-dRIS:
Maximum absorption wavelength (λmax (abs)): 493 nm
Maximum emission wavelength (λmax (em)): 518 nm
Extinction coefficient (M-1cm-1): 73,000 (0.1 M phosphate buffer, pH 7.2)
Spectroscopic properties of AF647-RISPC:
Maximum absorption wavelength (λmax (abs)): 648 nm
Maximum emission wavelength (λmax (em)): 666 nm
Extinction coefficient (M-1cm-1): 240,000 (0.1 M phosphate buffer, pH 7.0)
*For laboratory use only. These products are intended for animal research only and not for use in humans.